Journal: International Journal of Stem Cells

Article Title: Induced Pluripotent Stem Cells Derived CD71 ＋ CD235a ＋ Erythroblasts Were Increased by Sirtuin 1 Activator

doi: 10.15283/ijsc25040

Figure Lengend Snippet: Characterization of pluripotent stem cell markers in iPSCs. (A) Immunofluorescence staining showing qualitative expression of pluripotency markers SOX2 and TRA-1-60 in iPSC cell lines N7, N9, N11, and N12. Scale bar=200 μm. (B) qRT-PCR analysis of gene expression levels of pluripotency markers NANOG and OCT4 in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). (C) Immunofluorescence staining showing qualitative expression of the hematopoietic commitment marker IGF2 in the iPSC cell lines. Scale bar=200 μm. (D) qRT-PCR analysis of gene expression levels of the hematopoietic commitment marker IGF2 and its receptor IGF1R in the iPSC cell lines. Data are presented as mean±SEM from 3 independent experiments (n=3). iPSCs: induced pluripotent stem cells, IGF2: insulin-like growth factor II.

Article Snippet: The primary antibodies were rat anti-human SRY-box transcription factor 2 (SOX2) (Invitrogen, A24759), mouse anti-human tumor-related Antigen-1-60 (TRA-1-60) (Invitrogen, A24868), insulin-like growth factor II (IGF2) (Santa Cruz Biotechnology, sc-515805) and rabbit anti-human Runt-related transcription factor 1 (RUNX1) (Abcam, ab35962).

Techniques: Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Gene Expression, Marker

Journal: PLOS One

Article Title: Cellular repressor of E1A-stimulated genes 1 enhances skeletal muscle performance through the stimulation of muscle differentiation and Akt-mTOR signaling pathway activation

doi: 10.1371/journal.pone.0328485

Figure Lengend Snippet: (A) Western blotting for CREG1 was performed using the serum (1 µL) of wild type (WT) and Tg mice. (B-C) The expression of CREG1 in the soleus muscles of WT and Tg mice was examined using RT-PCR and western blot analysis. Relative levels of Creg1 mRNA (B) and CREG1 protein (C) in the soleus muscles. (D) Relative levels of insulin-like growth factor 2 receptor (IGF2R), phosphorylated Akt Ser 473 (pAkt), total Akt (Akt), phosphorylated mechanistic target of rapamycin (pmTOR) Ser 2448 and total mTOR (mTOR) in the soleus muscles. Data were normalized to those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (E) Western blotting for IGF2R, pAkt, Akt, pmTOR, mTOR and GAPDH in the soleus muscles. Representative images are shown. Data are presented as mean ± SEM; n = 4–6 per group. A student's t -test was performed; * P < 0.05, versus WT.

Article Snippet: The membranes were blocked for 1 h at room temperature in nonfat dry milk and then incubated overnight at 4 °C with the following antibodies: anti-CREG1 (ab233282; Abcam, Cambridge, UK), anti-Akt Ser 473 [9271; Cell Signaling Technology (CST), Danvers, MA, USA], anti-Akt (9272; CST), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2118; CST), anti- insulin-like growth factor 2 receptor (IGF2R) (14364; CST), anti- mTOR Ser 2448 (2971; CST), anti- mTOR (2972; CST), and anti-α/β-tubulin (2148; CST).

Techniques: Western Blot, Expressing, Muscles, Reverse Transcription Polymerase Chain Reaction